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Serum and thyroid hormones T3 and T4 regulate nerve growth factor mRNA levels in mouse L cells
Author(s) -
Wion Didier,
Barrand Plana,
Dicou Eleni,
Scott James,
Brachet Philippe
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80837-1
Subject(s) - hormone , thyroid hormones , medicine , endocrinology , thyroid , nerve growth factor , messenger rna , triiodothyronine , biology , gene , receptor , biochemistry
Mouse L cells synthesize and secrete a neurotrophic factor related to the β subunit of the submaxillary gland nerve growth factor (NGF) of male mice. Use of a cDNA probe which encodes the β‐NGF mRNA demonstrated that L cells produce a transcript identical in size to that of the submaxillary gland. Moreover, target sites of restriction enzymes Eco RI, Pst I and Bam HI were not significantly rearranged in the β‐NGF gene locus of these cells. The abundance of the β‐NGF transcript was found to depend on culture conditions. Removal of serum depressed the cellular content of polyadenylated RNA by a factor of 1.7, and decreased specifically the pool of β‐NGF transcript by an additional factor of 4. The presence of 10 −7 M testosterone in the serum‐free medium did not modify the level of β‐NGF mRNA, while addition of 10 −7 M T3 (or T4) increased this level by a factor of 1.5. These data provide the first evidence that the β‐NGF mRNA of L cells is subjected to regulation, but in a way apparently different from that described for the submaxillary gland.