Premium
Action of a cytotoxin from Pseudomonas aeruginosa on human leukemic cell lines
Author(s) -
Sasaki Terukatsu,
Lutz Frieder
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80836-x
Subject(s) - jurkat cells , inositol , cell culture , pseudomonas aeruginosa , chemistry , permeability (electromagnetism) , microbiology and biotechnology , fluorescence , pseudomonas , inositol phosphate , biochemistry , membrane , biology , bacteria , immunology , t cell , receptor , genetics , immune system , physics , quantum mechanics
Quin2 loaded human leukemic, JURKAT and K562 cells, were exposed to various doses of Pseudomonas aeruginosa cytotoxin. This cytotoxin induced an increase in quin2 fluorescence indicating an increase in the cytoplasmic free Ca 2+ concentration. The rate of the fluorescence increase and the lag time before the response were dependent on the doses of the cytotoxin. Addition of MnCl 2 to the cytotoxin‐treated cells induced a decrease in the quin2 fluorescence at rates dependent on the doses of the cytotoxin. The cytotoxin did not stimulate the inositol lipid turnover in JURKAT cells, which was determined by the accumulation of [ 3 H]inositol phosphates in myo ‐[2‐ 3 H]inositol‐prelabeled cells in the presence of LiCl. These results indicate that the cytotoxin increases cell permeability to both Ca 2+ and Mn 2+ by direct breakdown of the permeability barrier of the plasma membrane.