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Alkaline phosphatase of the calf intestine hydrolyzes phospholipids
Author(s) -
Ehle Heidrun,
Müller Elke,
Horn Anton
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80822-x
Subject(s) - phosphatidylinositol , phosphatidic acid , alkaline phosphatase , chemistry , phosphomonoesterase , hydrolysis , incubation , biochemistry , phosphatase , enzyme , pi , phosphodiesterase , acid phosphatase , phospholipid , phosphorylation , membrane
Pure alkaline phosphatase of the calf intestine is able to hydrolyze phosphatidylinositol 4,5‐diphosphate (TPI) to phosphatidylinositol and P i and to dephosphorylate phosphatidic acid. This phosphomonoesterase activity shows a considerably high specifie activity when an incubation medium at neutral pH containing 3 mM deoxycholate is used. The activity is inhibited by low concentrations of Ca 2+ . The enzyme has no detectable phosphodiesterase activity under the conditions tested.