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Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene
Author(s) -
Pasloske Brittan L.,
Finlay B.Brett,
Paranchych William
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80821-8
Subject(s) - pilin , gene , pseudomonas aeruginosa , cloning (programming) , genetics , biology , hindiii , amino acid , chromosome , pseudomonas , microbiology and biotechnology , restriction fragment , molecular cloning , peptide sequence , pilus , restriction enzyme , bacteria , escherichia coli , computer science , programming language
A 1.2‐kilobase (kb) Hind III restriction fragment containing the pilin gene from Pseudomonas aeruginosa PAK has been cloned and sequenced. The pilin protein is 144 amino acids in length with a positively charged leader sequence of 6 amino acids. There is probably only one copy of the gene per chromosome.