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Regulation of DNA ligation by the rec B protein in Escherichia coli cells
Author(s) -
Syväoja Juhani,
Pyhtilä Mauno J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80815-2
Subject(s) - dna ligase , escherichia coli , lysogen , dna , biochemistry , dna ligases , microbiology and biotechnology , ligation , chemistry , nad+ kinase , gene , biology , enzyme , bacteriophage
The covalent closing of hydrogen‐bonded λDNA circles in unpurified or slightly purified extracts of Escherichia coli , which was previously shown to require ATP and rec B gene products, was found not to require rec C gene product. The closing activity in extracts of the λ lysogen that overproduces DNA ligase about 500‐fold over the normal strain was still strictly dependent on ATP, but not on NAD. This suggests that the substrate was not freely available for the action of DNA ligase in rec B − extract. ATP could be replaced in the system by a mixture of low concentrations of the 4 dNTPs, but by none of them alone.