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Quantification of picogram levels of specific DNA immobilized in microtiter wells
Author(s) -
Nagata Yoshiho,
Yokota Hiroshi,
Kosuda Osamu,
Yokoo Keiko,
Takemura Kazuo,
Kikuchi Tetsuya
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80814-0
Subject(s) - biotinylation , microtiter plate , chemistry , chromatography , dna , hybridization probe , biochemistry
A non‐radioisotopic and sensitive method for quantification of specific DNA immobilized in microtiter wells has been developed. This method is based upon the immobilization of DNA in microtiter wells and hybridization with biotinylated DNA probe which is followed by complexing with avidin‐β‐galactosidase. By measuring fluorescence emitted from the hydrolyzed product by β‐galactosidase of 4‐methylumbelliferyl‐β‐D‐galactoside, it has become possible to quantify a few picograms of specific DNA in DNA samples immobilized in plastic microtiter wells.