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Biosynthesis of androgens and pheromonal steroids in neonatal porcine testicular preparations
Author(s) -
Kwan T.K.,
Orengo C.,
Gower D.B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80810-3
Subject(s) - microsome , pregnenolone , testosterone (patch) , medicine , biosynthesis , endocrinology , chemistry , cleavage (geology) , cofactor , cytosol , biochemistry , biology , steroid , enzyme , hormone , paleontology , fracture (geology)
The biosynthesis of testosterone and 4‐androstene‐3,17‐dione and some 16‐androstenes has been studied in homogenates or subcellular fractions of testes from 3‐week‐old Landrace piglets. Pregnenolone was converted into 5,16‐androstadien‐3β‐ol, 4,16‐androstadien‐3‐one, 5α‐androst‐16‐en‐3‐one and 5α‐androst‐16‐en‐3α‐ and 3β‐ols, but the quantities were some 50 times less than those formed in the mature boar testis. Androgens were also formed in the microsomal fractions but the quantities of 4‐androstene‐3,17‐dione (from side‐chain cleavage of 17‐hydroxyprogesterone) and of testosterone (from reduction of 4‐andro stene‐3,17‐dione) were 50–70 times lower than in the adult animal. The kinetic parameters and cofactor preference of the 3α‐ and 3β‐hydroxysteroid dehydrogenases were determined in the cytosolic, microsomal and mitochondrial fractions of neonatal porcine testes.