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Reconstitution of the epidermal growth factor receptor in artificial lipid bilayers
Author(s) -
Panayotou George N.,
Magee Anthony I.,
Geisow Michael J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80802-4
Subject(s) - liposome , epidermal growth factor receptor , epidermal growth factor , lipid bilayer , receptor , chemistry , biophysics , biochemistry , microbiology and biotechnology , membrane , biology
Epidermal growth factor receptor, isolated in the presence of the non‐ionic detergent octylglucoside, was reconstituted into liposomes by detergent dialysis. The receptor was shown to be incorporated in the lipid bilayer with the EGF binding domain oriented to the outside of the liposomes and to retain its ability to bind EGF and an anti‐receptor antibody in a specific manner.