Premium
Immunological quantitation of tyrosinase from wild‐type and albino mutant mice
Author(s) -
Tamate Hidetoshi B.,
Kuzumaki Takejiro,
Suzuki Jun-ichi,
Ishikawa Kiichi
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80793-6
Subject(s) - tyrosinase , locus (genetics) , mutant , enzyme , immunoelectrophoresis , wild type , microbiology and biotechnology , genotype , chemistry , gene , biology , biochemistry , genetics , antigen
The relationship between gene dosage, enzyme activity, and level of immunologically cross‐reacting material (CRM) was examined in mammalian tyrosinase (EC 1.14.18.1) by rocket immunoelectrophoresis. Skin extracts from mice heterozygous ( C/c ) and homozygous ( c/c ) for the albino locus contain 46% and 0% of CRM, respectively, as compared with wild‐type ( C/C ) animals. Enzyme activity and CRM level were directly proportional in these genotypes, suggesting that the albino locus controls the quantity of tyrosinase produced in melanocytes.