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3'‐Fluoro‐2',3'‐dideoxyribonucleoside 5'‐triphosphates: terminators of DNA synthesis
Author(s) -
Chidgeavadze Zurab G.,
Scamrov Andrei V.,
Beabealashvilli Robert Sh.,
Kvasyuk Eugenii I.,
Zaitseva Galina V.,
Mikhailopulo Igor A.,
Kowollik Gotthard,
Langen Peter
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80792-4
Subject(s) - chemistry , dna , dna synthesis , combinatorial chemistry , biochemistry
It is shown that dNTP(3'F) are terminators of DNA synthesis and may serve as very effective tools for DNA sequencing with E.coli DNA polymerase I and AMV reverse transcriptase. The dNTP(3'F) are found to be chain terminator substrates for calf thymus terminal deoxyribonucleotidyl transferase but not for calf thymus DNA polymerase α. The optimal dNTP(3'F) concentration for DNA sequencing by DNA polymerase I is found to be an order of magnitude lower than that of ddNTPs. dNTP(3'F) produce a more clear sequence pattern than do ddNTPs.