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Fractionation of chromatin fragments on columns of Biogel A50‐m at different salt concentrations
Author(s) -
Huang Hui-Chuan,
Cole R.David
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80791-2
Subject(s) - chromatin , fractionation , chemistry , histone , elution , salt (chemistry) , nuclease , biophysics , chromatography , dna , biochemistry , biology , organic chemistry
Nuclease fragmented chromatin was chromatographed on Biogel at various NaCl concentrations. The yield of eluted chromatin, and its H1/core histone ratio was minimal at 0.18 M NaCl where the ratio of H1 subtypes H1c/H1 ab was maximal. Therefore, the eluted material was aggregation‐resistant chromatin while aggregatable chromatin remained on the columns. Previous results were interpreted as Hl depletion of chromatin by ion‐exchange properties of Biogel, but the primary phenomenon is now seen as a separation of classes of chromatin that differ in sensitivity to salt‐induced aggregation. At very low salt concentrations, Biogel chromatography can be used without concern for H1 depletion.