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Carbon tetrachloride‐induced lipid peroxidation dependent on an ethanol‐inducible form of rabbit liver microsomal cytochrome P‐450
Author(s) -
Johansson Inger,
Ingelman-Sundberg Magnus
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80790-0
Subject(s) - carbon tetrachloride , lipid peroxidation , cytochrome , chemistry , microsome , ethanol , biochemistry , rabbit (cipher) , antioxidant , enzyme , organic chemistry , statistics , mathematics
Treatment of rats with ethanol or rabbits with either imidazole or pyrazole, agents known to induce the ethanol‐inducible form of liver microsomal cytochrome P‐450 (P‐450 LMeb), caused, compared to controls, 3–25‐fold enhanced rates of CCl 4 ‐dependent lipid peroxidation or chloroform production in isolated liver microsomes. No significant differences were seen when the rate of CCl 4 ‐dependent lipid peroxidation was expressed relative to the amount of P‐450 LMeb in the various types of microsomal preparations. In reconstituted membranous systems, this type of P‐450 was a 100‐fold more effective catalyst of CCl 4 metabolism than either of the cytochromes P‐450 LM 2 or P‐450 LM 4 . It is proposed that the induction of this isozyme provides the explanation on a molecular level for the synergism seen of ethanol on CCl 4 ‐dependent hepatotoxicity.