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Distribution of intrinsic and extrinsic subunits of the PS II protein complex between appressed and non‐appressed regions of the thylakoid membrane: an immunocytochemical study
Author(s) -
Vallon O.,
Wollman F.A.,
Olive J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80786-9
Subject(s) - thylakoid , spinach , photosystem ii , protein subunit , membrane , chemistry , tricine , biophysics , immunogold labelling , biology , biochemistry , photosynthesis , botany , chloroplast , ultrastructure , gene
The distribution of photosystem (PS) II centers between appressed and non‐appressed domains of the thylakoid membranes has been investigated, using immunogold labeling, in C. reinhardtii and in spinach. We have used antibodies directed against the two main intrinsic subunits of the PS II center and against two extrinsic subunits of the O 2 ‐evolving site. 90% of the two intrinsic PS II antigens are located in the appressed membranes, in a density 3–3.5‐fold higher than in the non‐appressed regions. The same distribution is observed for the two extrinsic antigens. This result suggests that the O 2 ‐evolving system is associated with the PS II center, both in appressed and in non‐appressed regions of the thylakoid membrane.