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Evidence for a Ca 2+ ‐independent hydrolysis of phosphatidylinositol 4,5‐bisphosphate in neuron‐like cell line NG108‐15 cells
Author(s) -
Yano Koh,
Higashida Haruhiro,
Nozawa Yoshinori
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80784-5
Subject(s) - phosphatidylinositol 4,5 bisphosphate , phosphatidylinositol , line (geometry) , chemistry , cell culture , hydrolysis , biochemistry , biology , genetics , signal transduction , geometry , mathematics
The addition of bradykinin to 32 P i ‐labeled neuroblastoma × glioma hybrid NG108‐15 cells caused a substantial loss of radioactivity from phosphatidylinositol 4,5‐bisphosphate (PI‐4,5‐P 2 ). The bradykinin‐induced hydrolysis of PI‐4,5‐P 2 was almost equally observed even when extracellular Ca 2+ was depleted with EGTA (100μM). On the other hand, high K + depolarization of the cells, which allows Ca 2+ influx through voltage‐dependent Ca 2+ channels, failed to induce any significant decrease in the radioactivity of PI‐4,5‐P 2 . These data indicate that the bradykinin‐stimulated PI‐4,5‐P 2 hydrolysis in NG108‐15 cells is independent of extracellular Ca 2+ and also that PI‐4,5‐P 2 hydrolysis is not stimulated by an elevation of intracellular Ca 2+ concentration.