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Interaction of plasmid DNA with yeast protoplasts and a mechanism of genetic transformation
Author(s) -
Brzobohatý Břetislav,
Kováč Ladislav
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80778-x
Subject(s) - protoplast , plasmid , transformation (genetics) , dna , saccharomyces cerevisiae , yeast , exogenous dna , biology , biophysics , plasmid preparation , microbiology and biotechnology , biochemistry , chemistry , gene , pbr322
DNA of plasmid pBB 29 is bound to protoplasts of Saccharomyces cerevisiae by a time‐dependent, energy‐independent process requiring Ca 2+ . It is not degraded during binding but most of the bound DNA can be degraded by exogenously added DNase. Foreign DNA competes for the binding. Genetic transformation of protoplasts depends on the same conditions as does the binding of DNA but, in addition, it also requires conditions allowing fusion of the protoplasts. Transformation may consist of firm adsorption of plasmid DNA to the protoplast surface, mediated by Ca 2+ , and of internalization of the DNA entrapped between two aggregated protoplasts as a suit of their fusion.