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The azido[ 14 C]atrazine photoaffinity technique labels a 34‐kDa protein in Scenedesmus which functions on the oxidizing side of photosystem II
Author(s) -
Metz James G.,
Bricker Terry M.,
Seibert Michael
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80768-7
Subject(s) - photosystem ii , atrazine , oxidizing agent , photoaffinity labeling , chemistry , mutant , biochemistry , stereochemistry , photosynthesis , biology , binding site , organic chemistry , pesticide , gene , agronomy
We have used azido[ 14 C]atrazine to photoaffinity label thylakoids from wild‐type (WT) Scenedesmus and a mutant, LF‐1, which is blocked on the oxidizing side of photosystem II (PS II). One protein is labeled in each case, at 34 kDa in the WT and 36 kDa in LF‐1. Previous comparison of the WT with LF‐1 had been used to assign a PS II donor side function to the 34‐kDa protein. These results suggest that this photoaffinity technique does not label the herbicide‐binding protein involved in electron transfer on the reducing side of PS II.