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Purification of liver and hepatoma membrane proteins by high‐performance liquid chromatography
Author(s) -
Josić Dj.,
Schuett W.,
Neumeier R.,
Reutter W.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80766-3
Subject(s) - chromatography , high performance liquid chromatography , membrane , chemistry , membrane protein , protein purification , biochemistry
This paper documents the recovery of selected proteins from hepatic plasma membranes. Initial purification, achieved by a series of stepwise extractions, facilitates the subsequent purification by HPLC. Examples are provided to illustrate the recovery of specific proteins from two Morris hepatoma lines and the liver.