Premium
C1q binding to mitochondria: a possible artefact?
Author(s) -
Comis Alfio,
Easterbrook-Smith Simon B.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80749-3
Subject(s) - mitochondrion , rnase p , pronase , ribonuclease , biochemistry , chemistry , microbiology and biotechnology , rna , trypsin , biology , enzyme , gene
Mitochondrial preparations, obtained from human tonsils and from rat spleen, liver, heart, and kidney tissues, bound [ 125 I]Clq with affinities of 10 7 –10 8 M −1 . The binding of Clq was not affected by treatment of the mitochondrial preparations with pronase, trypsin, or phospholipase D, but it was lowered 5–6‐fold following treatment of the mitochondria with DNase and RNase. Binding of Clq to mitochondrial preparations was also greatly diminished by limited chemical modification of Clq with cyclohexane‐1,2‐dione. It is suggested that the reported binding of Clq to mitochondria may have arisen from the protein binding to DNA and/or RNA contaminating the mitochondrial preparations.