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Relaxation of Pvu II recognition sequence
Author(s) -
Nasri Moncef,
Sayadi Sami,
Thomas Daniel
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80748-1
Subject(s) - pbr322 , restriction enzyme , dimethyl sulfoxide , recognition sequence , endonuclease , dna , substrate specificity , sulfoxide , sequence (biology) , genetics , chemistry , relaxation (psychology) , microbiology and biotechnology , stereochemistry , biology , biochemistry , computational biology , enzyme , plasmid , organic chemistry , neuroscience
The substrate specificity of Pvu II endonuclease is relaxed in the presence of dimethyl sulfoxide. The new recognition sequences cleaved in pBR322 DNA have been found to be CCGCTG, CATCTG, CAGATG, CAGGTG and CAGCGG.