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Topography of RNA in the ribosome: location of the 5 S RNA residues A 39 and U 40 on the central protuberance of the 50 S subunit
Author(s) -
Evstafieva A.G.,
Shatsky I.N.,
Bogdanov A.A.,
Vasiliev V.D.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80740-7
Subject(s) - ribosome , rna , protein subunit , ribosomal rna , crystallography , chemistry , position (finance) , eukaryotic ribosome , microbiology and biotechnology , biophysics , biology , biochemistry , gene , finance , economics
The internal site of 5 S RNA comprising residues A 39 and U 40 has been localized on the E.coli 50 S ribosomal subunit by immune electron microscopy. It has been found to be located on the interface side of the central protuberance at the position distinctly apart but very close to the position of the 5 S RNA 3'‐end providing evidence for a quite compact folded conformation of the 5 S RNA in situ.