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Glutamate‐glutamine metabolism in the perfused rat liver
Author(s) -
Desmoulin F.,
Canioni P.,
Cozzone P.J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80734-1
Subject(s) - glutaminase , glutamine , glutamate receptor , metabolism , glutamine synthetase , biochemistry , cytosol , glutamate dehydrogenase , biology , glutamate synthase , glutaminolysis , amino acid , enzyme , receptor
13 C‐NMR has been used te follow the metabolism of 13 C‐enriched substrates in isolated perfused rat liver. The fate of 90% enriched [2‐ 13 C]acetate has been studied in the perfused liver in order to investigate mitochondrial metabolism and the interrelations between cytosolic and mitochondrial pools of metabolites. Some compounds of the intermediary metabolism where found to be extensively labelled, e.g. glutamate, glutamine, acetoacetate and β‐hydroxybutyrate. Under our experimental conditions, labelling of glutamate reached a steady‐state within 30 min after the onset of perfusion of 20 mM acetate. In addition, the observed incorporation of 13 C into glutamine can be linked to the operation of the glutamate‐glutamine antiport and to the high activity of cytosolic glutamate synthetase. The finding of both active glutaminase and glutamine synthetase activity in the same liver cells is evidence of the existence of an active glutamine‐glutamate futile cycle.