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1α,25‐Dihydroxy vitamin D 3 (calcitriol) stimulates proliferation of human circulating monocytes in vitro
Author(s) -
Ohta Masataka,
Okabe Tetsuro,
Ozawa Keiya,
Urabe Akio,
Takaku Fumimaro
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80730-4
Subject(s) - calcitriol , in vitro , monocyte , vitamin d and neurology , lectin , microbiology and biotechnology , medicine , endocrinology , cell culture , chemistry , thymidine , cell growth , calcitriol receptor , mitosis , biology , biochemistry , immunology , genetics
Previous studies demonstrated that human circulating monocytes can proliferate in vitro when incubated with lectin‐induced factor(s) from lymphocytes [(1985) Biochem. Biophys. Res. Commun., in press]. This study shows that human monocytes were induced to proliferate when incubated with 1α,25‐dihydroxy vitamin D 3 (calcitriol) at physiological concentrations. The optimal dose was about 10 nM. Proliferative activity was examined both by measuring the [su3H]thymidine incorporation and by counting cell nuclei. Among other derivatives of vitamin D 3 , 1α,24 R ‐dihydroxyvitamin D 3 and 1α,24 R ,25‐trihydroxyvitamin D 3 stimulated mitotic activity of monocytes. Addition of both calcitriol and lectin‐stimulated lymphocyte‐conditioned medium to the monocyte culture had an additional effect on the mitotic activity of monocytes.