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Rapid modulation of transcription of nuclear genes encoding chloroplast proteins by light
Author(s) -
Gallagher T.F.,
Jenkins G.I.,
Ellis R.J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80716-x
Subject(s) - transcription (linguistics) , gene , nuclear gene , biology , complementary dna , rubisco , chloroplast dna , transcription factor , chloroplast , microbiology and biotechnology , genetics , genome , philosophy , linguistics
We have investigated the effects of light on transcription of the nuclear genes encoding the small subunit (SSU) of ribulose‐1,5‐biphosphate carboxylase‐oxygenase, and the major apoprotein (LHCP) of the lightharvesting chlorophyll a / b complex in Pisumsativum . Light treatments were given in vivo and transcription was assayed subsequently in isolated nuclei using specific cloned cDNA probes. We have identified three different temporal effects of light on transcription of these genes: an initial increase when dark‐grown seedlings are first illuminated, a slow increase in the ability to transcribe these genes at maximal rates, and a rapid modulation of specific gene transcription in fully greened plants.