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Further characterization of cardiodigin, Na + K + ‐ATPase inhibitor extracted from mammalian tissues
Author(s) -
Fagoo M.,
Godfraind T.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80672-4
Subject(s) - digoxin , high performance liquid chromatography , endogeny , chemistry , digitalis , atpase , chromatography , column chromatography , biochemistry , enzyme , medicine , heart failure
This study was undertaken to characterize endogenous digitalis‐like activity in water extract from mammalian tissues. Prepurified samples obtained from guinea‐pig heart were analysed by reverse‐phase HPLC using an acetonitrile gradient. The eluent was assayed for its activity as inhibitor of human heart Na + , K + ‐ATPase and digoxin‐like immunoreactivity. Both activities were recovered in the same fraction after two successive chromatographic steps. These results provide further evidence for the presence of an endogenous digitalislike factor, cardiodigin, in mammalian heart.