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The chloroplast 32 kDa protein is synthesized on thylakoid‐bound ribosomes in Chlamydomonas reinhardtii
Author(s) -
Herrin David,
Michaels Allan
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80660-8
Subject(s) - thylakoid , reticulocyte , chlamydomonas reinhardtii , polysome , biochemistry , chlamydomonas , chloroplast , ribosome , lysis , biology , protein biosynthesis , chloroplast dna , microbiology and biotechnology , messenger rna , rna , gene , mutant
A cloned cpDNA fragment containing a portion of the gene for the 32–36 kDa thylakoid protein of Chlamydomonas (polypeptide D‐1) was isolated. Hybridization probing of RNA from soluble and membrane fractions of Chlamydomonas showed that the mRNA for D‐1 is bound to thylakoid membranes. Run‐off translation of thylakoid‐bound polysomes (rough thylakoids) with [ 35 S]‐methionine yields polypeptide D‐1 as the major product. Peptide mapping with S. aureus V‐8 protease of D‐1 synthesized (1) in vivo, (2) in vitro by rough thylakoids and (3) in the reticulocyte lysate directed by non‐polyadenylated RNA showed that D‐1 is synthesized as a precursor in the reticulocyte lysate but as the mature polypeptide by rough thylakoids.