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Growth‐related variation of α 2 ‐adrenergic receptivity in the HT 29 adenocarcinoma cell‐line from human colon
Author(s) -
Paris H.,
Bouscarel B.,
Cortinovis C.,
Murat J.C.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80658-x
Subject(s) - receptivity , colorectal adenocarcinoma , cell culture , adrenergic , medicine , adenocarcinoma , variation (astronomy) , line (geometry) , endocrinology , biology , chemistry , genetics , receptor , mathematics , cancer , physics , geometry , astrophysics
The human colon adenocarcinoma cell‐line HT 29 has been shown to possess functional α 2 ‐adrenergic receptors. Here, [ 3 H] clonidine was used as radioligand to study the evolution of α 2 ‐adrenergic receptivity during the time course of HT 29 cell culture. Scatchard analysis of the saturation curves indicates that the number of [ 3 H]clonidine binding sites increases throughout the 17 day culture period. The maximal number of α 2 adrenoceptors is found during the stationary phase of growth, when cell density is high and mitotic rate low. Moreover, the use of adrenaline and clonidine as α 2 ‐adrenergic agonists reveals a relationship between the number of receptors and the intensity of the biological effect associated with their stimulation (inhibition of the VIP‐induced cyclic AMP accumulation).