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Characterization and crystallization of ribosomal particles from Halobacterium marismortui
Author(s) -
Shevack A.,
Gewitz H.S.,
Hennemann B.,
Yonath A.,
Wittmann H.G.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80655-4
Subject(s) - halobacterium , halophile , ribosome , crystallization , ribosomal rna , crystallography , chemistry , salt (chemistry) , electron microscope , halobacteriaceae , biophysics , bacteria , biology , biochemistry , archaea , physics , halobacterium salinarum , organic chemistry , optics , genetics , rna , gene
Ribosomes and their subunits have been isolated from Halobacterium marismortui , an extremely halophilic bacterium from the Dead Sea. The stability and functional activity of the subunits were tested under a wide range of salt conditions. Three‐dimensional microcrystals of the large ribosomal subunits have been obtained. Electron microscopy of positively stained thin sections of these crystals showed that the particles are closely packed with approximate cell constants of 310 × 350 Å.