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Stimulation of the respiratory and phosphorylating activities in rat brain mitochondria by idebenone (CV‐2619), a new agent improving cerebral metabolism
Author(s) -
Sugiyama Yasuo,
Fujita Takeshi
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80650-5
Subject(s) - respiration , idebenone , mitochondrion , stimulation , respiratory system , metabolism , biology , oxygen , biochemistry , respiratory chain , endocrinology , medicine , chemistry , anatomy , organic chemistry
The effects of 6‐(10‐hydroxydecyl)‐2,3‐dimethoxy‐5‐methyl‐l,4‐benzoquinone (CV‐2619) on the respiration and non‐respiratory oxygen consumption induced by ascorbate and Fe 2+ in rat brain mitochondria were studied. When CV‐2619 (100 and 300 ) was orally administered to rats for 3 days, it increased the state 3 respiration stimulated by ADP, slightly decreased the state 4 respiration after the consumption of ADP and resulted in a significant increase of the respiratory control index (RCI) by 14–19% for glutamate oxidation( P <0.01) and 10–17% for succinate oxidation ( P <0.05), respectively. The RCI increasing effect of CV‐2619 was dose dependent, but the compound had no effect on the ADP/O ratio. CV‐2619 significantly suppressed by about 10% the non‐respiratory oxygen consumption ( P <0.02), which closely associated with non‐enzymatic reactions such as lipid peroxidation, membrane lysis and swelling of mitochondria. Thus, CV‐2619 may contribute to stimulate the net ATP formation by the well‐coupling of electron and energy transfer, and by the reduction of non‐respiratory oxygen consumption in cerebral metabolism.