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Incorporation of isoleucine into protein by a soluble fraction from spermatozoa
Author(s) -
Hashizume Shuichi
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80649-9
Subject(s) - isoleucine , fraction (chemistry) , chemistry , chromatography , biochemistry , leucine , amino acid
A heat‐labile, non‐dialyzable factor(s) in soluble fractions from porcine, bull, rabbit and cock spermatozoa was found to incorporate the radioactivity of [ 14 C]isoleucine into a 95°C CCl 3 COOH‐insoluble fraction. The incorporation required ATP, Mg 2+ , casein and 2‐mercaptoethanol. Trypsin and a‐chymotrypsin inhibited the incorporation, while RNase A and DNase I did not. A mixture of 19 amino acids other than isoleucine had no effect on the incorporation. The reaction product was identified as protein. The incorporated moiety was the isoleucyi moiety of isoleucine and it retained a free α‐amino group in the product protein. Some other characteristics of this incorporation are also described.