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Apparent inhibition of glycoprotein synthesis by S.cerevisiae mating pheromones
Author(s) -
Orlean Peter,
Arnold Elisabeth,
Tanner Widmar
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80629-3
Subject(s) - glucosamine , glycoprotein , saccharomyces cerevisiae , mating , biochemistry , endogeny , sex pheromone , chitin , chemistry , biology , microbiology and biotechnology , yeast , genetics , chitosan
Saccharomyces cerevisiae mating pheromones a and α factor strongly inhibit the incorporation of radio‐labelled glucosamine into N ‐glycosylated proteins of corresponding haploid cells. This observation was erroneously interpreted as an inhibition of glycoprotein synthesis. It has turned out that α factor causes a 4–5‐fold dilution of incorporated [ 14 C]glucosamine with non‐radioactive endogenous precursor. In the case of the [ 14 C]chitin synthesized, which does not show inhibition by α factor, the lowering of the specific activity of the precursor is exactly compensated for by an increased rate of chitin synthesis caused by α factor.