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Increased level of calcitonin mRNA after 1,25‐dihydroxyvitamin D 3 injection in the rat
Author(s) -
Segond N.,
Legendre B.,
Tahri E.H.,
Besnard P.,
Jullienne A.,
Moukhtar M.S.,
Garel J.-M.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80620-7
Subject(s) - endocrinology , medicine , calcitonin , messenger rna , gene expression , chemistry , vitamin d and neurology , radioimmunoassay , western blot , microbiology and biotechnology , biology , gene , biochemistry
Vitamin D metabolites are able to change plasma calcitonin (CT) levels, but nothing is known about a possible effect at the CT gene level. Here we have investigated the acute effects of 1,25‐dihydroxyvitamin D 3 (1,25‐(OH) 2 D 3 on the CT biosynthetic activity of thyroid glands from adult rats. Plasma CT levels were significantly increased (× 2) 1 and 2 h after 1,25‐(OH) 2 D 3 injection in the face of unchanged plasma calcium values. The thyroidal CT content also was unchanged. A 2‐fold increase in CT mRNA level measured by dot‐blot hybridization occurred 1 and 2 h after 1,25‐(OH) 2 D 3 administration. Expression of CT gene products was examined in the rabbit reticulocyte lysate cell‐free translation assay. After polyacrylamide gel electrophoresis, specific immunoprecipitates were autoradiographed and quantified by integration. A single precursor of M r ≊ 15000 could be specifically immunoprecipitated with CT antisera. A 3–4‐fold rise in translatable CT mRNA activity was observed 1 and 2 h after 1,25‐(OH) 2 D 3 injection. Thus, parallel changes in CT mRNA level, CT mRNA activity and plasma CT levels were observed in adult female rats after administration of 1,25‐(OH) 2 D 3 . These findings demonstrate for the first time that 1,25‐(OH) 2 D 3 enhanced CT gene expression in the face of unchanged plasma calcium levels.