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A low‐ M r chemoattractant for vascular endothelial cells
Author(s) -
Büki Kálmán G.,
Seppä Heikki
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80617-7
Subject(s) - chemotaxis , endothelial stem cell , chemistry , microbiology and biotechnology , biochemistry , biology , in vitro , receptor
The formation of new blood vessels occurs by sprouting from previously existing microvasculature. The process involved directed migration of the vascular endothelial cells towards chemical signals released from the target tissue. We have used the Boyden chemotaxis chamber method to identify chemotactic signals for fetal bovine vascular endothelial cells. Human placenta organ cultures produce a high‐ M r chemoattractant for the endothelial cells from which a low‐ M r factor can be liberated with trichloroacetic acid treatment and ethanol extraction. This activity was isolated from extracts of human placenta using Sephadex LH‐20, Amberlite XAD‐2, and silica gel thin‐layer chromatography. The M r of the factor is less than 400, it is lipophilic and resistant to proteolytic enzymes. The factor induces chemotactic migration of both aortic endothelial cells and capillary endothelial cells from the retina, but has no effect on fibroblasts or leukocytes suggesting a specific function of the compound for the vascular endothelial cells.