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Determination of the amount and the type of quinones present in single crystals from reaction center protein from the photosynthetic bacterium Rhodopseudomonas viridis
Author(s) -
Gast P.,
Michalski T.J.,
Hunt J.E.,
Norris J.R.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80544-5
Subject(s) - photosynthetic reaction centre , quinone , crystallization , chemistry , purple bacteria , rhodospirillaceae , rhodopseudomonas , bacteria , mass spectrometry , center (category theory) , photosynthesis , rhodospirillales , photochemistry , stereochemistry , crystallography , biochemistry , organic chemistry , biology , chromatography , genetics
The identity and amount of quinone in single crystals from reaction center protein from Rhodopseudomonas viridis have been determined using HPLC, optical spectroscopy and mass spectrometry. The ratio menaquinone‐9/ubiquinone‐9 for solutions of reaction center protein of Rps. viridis was 0.93 ± 0.05. After crystallization of the reaction center solution, the quinone ratio was 1.6 ± 0.1. These observations show that about half of the ubiquinone‐9 can be lost during crystallization.