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Nucleotide sequences of cDNAs encoding precursors of human insulin‐like growth factor II (IGF‐II) and an IGF‐II variant
Author(s) -
Jansen M.,
van Schaik P.M.A.,
van Tol H.,
Van den Brande J.L.,
Sussenbach J.S.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80527-5
Subject(s) - complementary dna , signal peptide , peptide sequence , biology , nucleic acid sequence , microbiology and biotechnology , coding region , alternative splicing , amino acid , peptide , gene , genetics , messenger rna , biochemistry
We have isolated 3 cDNA clones encoding human IGF‐II and a variant of IGF‐II. The amino acid sequence encoded by the IGF‐II cDNA is identical to the sequence previously described [(1978) FEBS Lett. 89, 283‐286]. In the amino acid sequence predicted by the IGF‐II variant cDNA, the Ser residue 29 in the B‐domain has been replaced by an Arg‐Leu‐Pro‐Gly sequence. The corresponding mRNAs probably arise by alternative splicing of a common RNA precursor. The IGF coding region of the cDNA inserts is flanked by sequences encoding a signal peptide and a carboxy‐terminal peptide indicating that both human IGF‐II and its variant are synthesized as precursors.