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Measurement by radioimmunoassay of the mitochondrial uncoupling protein from brown adipose tissue of obese (ob/ob) mice and Zucker (fa/fa) rats at different ages
Author(s) -
Ashwell Margaret,
Holt Susan,
Jennings Graham,
Stirling Dorothy M.,
Trayhurn Paul,
York David A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80525-1
Subject(s) - radioimmunoassay , brown adipose tissue , uncoupling protein , adipose tissue , endocrinology , medicine , thermogenin , chemistry , biology
The concentration of the ‘uncoupling protein’ in brown adipose tissue mitochondria has been measured in lean and obese (ob/ob) mice and Zucker (fa/fa) rats at different ages using a specific radioimmunoassay. During the suckling period the concentration of the protein was similar in normal and mutant animals of both types, despite the decrease in mitochondrial GDP binding observed in the obese. The concentration of uncoupling protein was, however, decreased in adult ob/ob mice and adult Zucker rats compared with their respective lean siblings, in parallel with the decrease in GDP binding. It is concluded that there is a ‘masked’, or inactive, form of uncoupling protein in young ob/ob mice and fa/fa rats.