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Mononuclear cell supernatants inhibit prolyl hydroxylation
Author(s) -
Narayanan A.S.,
Meyers D.F.,
Page R.C.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80524-x
Subject(s) - hydroxylation , peripheral blood mononuclear cell , chemistry , secretion , biochemistry , fibroblast , type i collagen , in vitro , microbiology and biotechnology , enzyme , biology , endocrinology
The effect of phytohemagglutinin(PHA)‐activated human peripheral mononuclear cell supernatant (AS) on collagen production by human fibroblasts was examined. The AS inhibited collagen production in a doseand time‐dependent manner. Labeling and pulse chase experiments showed that it did not block collagen secretion, but a greater proportion of molecules synthesized in its presence accumulated within the cells. Amino acid analysis showed that when labeling was done at 24°C prolyl hydroxylation in fibroblasts exposed to the AS was reduced to two‐thirds of the cultures treated with control supernatant (CS), however it was not different at 37°C. These results indicate that the AS inhibits collagen hydroxylation, that the un(under)hydroxylated collagen molecules are degraded at physiological temperature and that suppression of collagen hydroxylation may be a mechanism by which the AS inhibits collagen production.