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Stereoelectronic effects in RNase‐catalysed reactions of dinucleoside phosphate cleavage
Author(s) -
Yakovlev Gennady I.,
Bocharov Alexander L.,
Moiseyev Gennady P.,
Mikhaylov Sergey N.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80521-4
Subject(s) - phosphodiester bond , moiety , rnase p , chemistry , rnase h , cleavage (geology) , nucleoside , stereochemistry , phosphate , nucleotide , substrate (aquarium) , biochemistry , rna , biology , paleontology , ecology , fracture (geology) , gene
The rate at which dinucleoside phosphates are cleaved by RNases is supposed to be determined by the mole fraction of enzyme‐substrate complexes in which the phosphodiester moiety of a dinucleoside phosphate has a highly reactive conformation. The mole fraction of such complexes for a particular RNase depends on the nature of a nucleoside at the O5'‐end of the phosphodiester bond. Experimental data are presented to support this hypothesis.