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Probing the three‐dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements
Author(s) -
Clore G.Marius,
Gronenborn Angela M.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80516-0
Subject(s) - nuclear overhauser effect , oligonucleotide , chemistry , proton , rna , dna , nuclear magnetic resonance spectroscopy , two dimensional nuclear magnetic resonance spectroscopy , proton nmr , nuclear magnetic resonance spectroscopy of nucleic acids , nuclear magnetic resonance , fluorine 19 nmr , stereochemistry , transverse relaxation optimized spectroscopy , physics , biochemistry , quantum mechanics , gene
DNA and RNA oligonucleotides are ideally suited for high‐resolution X‐ray crystallographic and 1 H‐NMR studies. The solution structures of such oligonucleotides can potentially be solved using proton‐proton nuclear Overhauser enhancement measurements to demonstrate the proximity of protons in space and to determine their separation, thereby enabling a comparison of the structure in the crystalline and solution states to be made. In this review we describe (i) the general strategy for the sequential resonance assignments of oligonucleotide 1 H‐NMR spectra, the essential prerequisite for further structural work, (ii) the approach to obtaining interproton distances from pre‐steady state nuclear Overhauser enhancement measurements, and (iii) the use of interproton distances in structure determination. This is illustrated by several examples including double‐ and single‐stranded DNA oligonucleotides as well as RNA stem and loop structures.