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A monoclonal antibody specific for the duplex DNA poly[d(TC)]· poly[d(GA)]
Author(s) -
Lee Jeremy S.,
Latimer Laura J.P.,
Woodsworth Mary L.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80440-3
Subject(s) - monoclonal antibody , duplex (building) , microbiology and biotechnology , virology , chemistry , dna , antibody , biology , genetics , biochemistry
Although most duplex DNAs are not immunogenic some synthetic DNAs such as polyl[d(Tm 5 C)] · poly‐[d(GA)] are weakly immunogenic allowing the production of monoclonal antibodies. The specificity of one of these antibodies, Jel 172, was investigated in detail by a competitive solid‐phase radioimmune assay. Jel 172 bound well to poly[d(TC)]·poly[d(GA)] but not to other duplex DNAs such as poly[d(TTC)] · poly‐[d(GAA)] and poly[d(TCC)]· poly[d(GGA)]. The binding to poly[d(Br 5 UC)]· poly[d(GA)] was enhanced while that to poly[d(TC)]· poly[d(IA)] was decreased compared to poly[d(TC)]· poly[d(GA)]. Thus, not only is the antibody very specific for a sequence of duplex DNA but it also appears to recognize functional groups in both grooves of the helix.