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Glycogen synthase (casein) kinase‐1: tissue distribution and subcellular localization
Author(s) -
Singh Toolsee J.,
Huang Kuo-Ping
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80433-6
Subject(s) - cytosol , glycogen phosphorylase , mitochondrion , subcellular localization , skeletal muscle , organelle , biochemistry , biology , glycogen , microsome , glycogen synthase , adipose tissue , phosphorylase kinase , enzyme , microbiology and biotechnology , cytoplasm , endocrinology
The distribution of glycogen synthase (casein) kinase‐1 (CK‐1) among different rat tissues and subcellular fractions was investigated. Using casein, glycogen synthase and phosphorylase kinase as substrates, CK‐1 activity was detected in kidney, spleen, liver, testis, lung, brain, heart, skeletal muscle and adipose tissue. The distribution of CK‐1 among different subcellular fractions of rat liver was; cytosol (72.1%), microsome (17.6%), mitochondria (9.6%) and nuclei (0.7%). CK‐1 from rat tissues was shown to have a similarly wide substrate specificity as highly purified CK‐1 from rabbit skeletal muscle. Such wide substrate specificity and distribution among different mammalian tissues and subcellular organelles indicate that CK‐1 may be involved in the regulation of diverse cellular functions.