Premium
Three‐dimensional structure of (Na + + K + )‐ATPase revealed by electron microscopy of two‐dimensional crystals
Author(s) -
Ovchinnikov Yu.A.,
Demin V.V.,
Barnakov A.N.,
Kuzin A.P.,
Lunev A.V.,
Modyanov N.N.,
Dzhandzhugazyan K.N.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80430-0
Subject(s) - crystallography , electron microscope , chemistry , crystal structure , resolution (logic) , homogeneous , physics , optics , artificial intelligence , computer science , thermodynamics
Prolonged incubation of membrane fragments containing homogeneous (Na + + K + )‐ATPase with Mg 2+ , K + and VO 3 − at 4°C resulted in formation of two‐dimensional crystals of this enzyme with unit cell parameters: a = 66 Å, b = 118 Å, γ = 108°. The crystals correspond to the two‐sided plane group p21. By combining tilted electron microscopic views of the crystals, a three‐dimensional structure of (Na + + K + )‐ATPase was calculated at ~20 Å resolution. The unit cell is formed by two (αβ)‐promoters which are in contact in their central parts. The structure was compared with chemical modification and immunochemical data; the arrangement of intra‐ and extramembrane domains was proposed.