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Transient deprotonation of bacterial halorhodopsin by photoexcited base
Author(s) -
Gutman M.,
Nachliel E.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80420-8
Subject(s) - deprotonation , halorhodopsin , chromophore , photochemistry , chemistry , halobacteriaceae , bacteriorhodopsin , excited state , proton , transient (computer programming) , atomic physics , physics , organic chemistry , biochemistry , ion , halobacterium salinarum , quantum mechanics , membrane , computer science , operating system
The excited singlet state of 6‐methoxyquinoline is basic enough to abstract a proton from water and generate OH − . Pulse excitation of such a solution undergoes a massive transient alkalinization. This procedure was employed to monitor the deprotonation of the Schiff base of halorhodopsin. Both the deprotonation and reprotonation of the chromophore are diffusion controlled reactions.