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Magnesium‐dependent conformational changes of membrane proteins are related to the Mg 2+ ‐dependent ATPase activity in cardiac sarcolemma
Author(s) -
Vrbjar N.,
Soos J.,
Ziegelhöffer A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80406-3
Subject(s) - sarcolemma , atpase , chemistry , biophysics , magnesium , membrane , cardiac muscle , biochemistry , enzyme , biology , endocrinology , organic chemistry
Magnesium‐induced enzymatic and structural changes of membrane‐bound proteins in rat heart sarcolemma have been investigated. In the absence of ATP, increasing concentrations of magnesium within the range 0.1–10.0 mM gradually lowered the α‐helix content of sarcolemmal proteins. The same magnesium concentrations stepwise activated the Mg 2+ ‐dependent ATPase in the presence of ATP. Mathematical and graphical analysis of the data yielded a quantitative relationship between magnesium‐induced stimulation of the Mg 2+ ‐dependent ATPase activity and diminution of the α‐helix content of membrane proteins in cardiac sarcolemma.