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Expression of plasmid encoded Escherichia coli 5 S ribosomal ribonucleic acid in Pseudomonas putida
Author(s) -
Hartmann Roland K.,
Henze Peter P.,
Ulbrich Norbert,
Erdmann Volker A.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80390-2
Subject(s) - pseudomonas putida , escherichia coli , plasmid , ribosomal rna , pseudomonas , microbiology and biotechnology , chemistry , biology , bacteria , biochemistry , genetics , gene
The recombinant plasmid pNRK 36, which represents the plasmids RSF 1010, a small multicopy plasmid of the incompatability group IncQ that confers resistance to streptomycin and sulfoamide to its host cells, and pKK‐ 223‐3, which contains the try‐lac (tac) promoter followed by a polylinker and a DNA segment containing the 5 S rRNA (rrn B) with the ribosomal RNA transcription terminators, was employed to transform Pseudomonas putida 2440 cells. The plasmid encoded 5 S rRNA from Escherichia coli was transcribed and processed properly in P. putida cells thus demonstrating for the first time the expression of a plasmid encoded ribosomal RNA in a heterologous system. The fact that the E. coli 5 S rRNA was not incorporated into assembled ribosomes suggests that the in vivo incorporation of 5 S rRNA into the 50 S ribosomal sub‐unit is closely linked to 23 S rRNA and/or ribosomal protein synthesis.