Premium
Occurrence and location of 7‐methylguanine residues in small‐subunit ribosomal RNAs from eubacteria, archaebacteria and eukaryotes
Author(s) -
Zueva Vera S.,
Mankin Alexander S.,
Bogdanov Alexei A.,
Thurlow David L.,
Zimmermann Robert A
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80378-1
Subject(s) - ribosomal rna , thermoplasma acidophilum , rna , biology , archaea , protein subunit , cleavage (geology) , guanine , genetics , biochemistry , nucleotide , bacteria , gene , enzyme , paleontology , fracture (geology)
Specific cleavage with aniline provides a rapid and convenient method for establishing the presence and approximate location of 7‐methyl‐guanine (m 7 G) residues in ribosomal RNA (rRNA) molecules. Using this approach, we have shown that a single m 7 G occurs roughly 465 bases from the 5'‐end of 16 S rRNA from the archaebacterium. Thermoplasma acidophilum , but that this modified base is absent from several other archaebacterial 16 S rRNAs. We have also demonstrated that a unique m 7 G is found some 220–230 bases from the 3'‐terminus of a number of eukaryotic 18 S rRNAs. In both cases, m 7 G is present within evolutionarily conserved structural features, suggesting that this base may optimize the activity of functionally important regions of rRNAs in a kingdom‐specific fashion.