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Prediction of sequential antigenic regions in proteins
Author(s) -
Welling Gjalt W.,
Weijer Wicher J.,
van der Zee Ruurd,
Welling-Wester Sytske
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80374-4
Subject(s) - antigenicity , antigen , cholera toxin , protein subunit , ribonuclease , glycoprotein , epitope , biology , peptide sequence , chemistry , biochemistry , computational biology , genetics , rna , microbiology and biotechnology , gene
Prediction of antigenic regions in a protein will be helpful for a rational approach to the synthesis of peptides which may elicit antibodies reactive with the intact protein. Earlier methods are based on the assumption that antigenic regions are primarily hydrophilic regions at the surface of the protein molecule. The method presented here is based on the amino acid composition of known antigenic regions in 20 proteins which is compared with that of 314 proteins [(1978) Atlas of Protein Sequence and Structure, vol. 5, suppl. 3, 363‐373]. Antigenicity values were derived from the differences between the two data sets. The method was applied to bovine ribonuclease, the B‐subunit of cholera toxin and herpes simplex virus type 1 glycoprotein D. There was a good correlation between the predicted regions and previously determined antigenic regions.