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Size and domain structure of collagen VI produced by cultured fibroblasts
Author(s) -
Aumailley Monique,
von der Mark Helga,
Timpl Rupert
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80362-8
Subject(s) - fibroblast , collagenase , pepsin , chemistry , molecular mass , collagen, type i, alpha 1 , disulfide bond , biochemistry , monomer , collagen vi , cell culture , microbiology and biotechnology , enzyme , biology , in vitro , extracellular matrix , organic chemistry , polymer , genetics
Disulfide‐bonded forms of collagen VI were analyzed by immunoblotting of fibroblast culture medium and cell extracts. The protein consists of pepsin and collagenase‐resistant domains of about equal size indicating a molecular mass of 340 kDa for collagen VI monomers.