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Specific immunolysis of serotonergic nerve terminals using an antiserum against tryptophan hydroxylase
Author(s) -
Docherty Maureen,
Bradford H.F.,
Cash Christopher D.,
Maitre Michel
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80360-4
Subject(s) - antiserum , tryptophan hydroxylase , serotonergic , lysis , tryptophan , chemistry , cholinergic , serotonin , endocrinology , medicine , biochemistry , antibody , biology , amino acid , immunology , receptor
An antiserum to tryptophan hydroxylase purified from whole rat brain when incubated with rat striatal synaptosomes in the presence of complement caused release of 18% of LDH, 20% loss of potassium and 60% loss of tryptophan hydroxylase. Uptake of 5‐HT was reduced by 60%. Anti‐tryptophan hydroxylase alone, or complement alone were without action. The antiserum plus complement had no effect on DA uptake and did not release TH or GAD. These results suggest selective lysis of serotonergic nerve terminals had occurred. The antiserum plus complement reduced choline uptake by 45%. However, this did not seem due to lysis of cholinergic terminals, as ChAT was not released.