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Periplasmic location of p ‐cresol methylhydroxylase in Pseudomonas putida
Author(s) -
Hopper David J.,
Jones Michael R.,
Causer Michael J.
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80359-8
Subject(s) - pseudomonas putida , periplasmic space , chemistry , pseudomonas , microbiology and biotechnology , biochemistry , biology , bacteria , genetics , escherichia coli , gene
The cellular location of the flavocytochrome c , p ‐cresol methylhydroxylase was investigated in two strains of Pseudomonas putida . In both cases the enzymes were shown to be located in the periplasmic fraction by their release during treatment of the bacteria with EDTA and lysozyme in a solution containing a high concentration of sucrose. For strain NCIB 9869 the finding is in accord with the suggestion that the physiological acceptor for the enyme is azurin as this too was shown to be located mostly in the periplasm.