Premium
The complete amino acid sequence of the major mammalian neurofilament protein (NF‐L)
Author(s) -
Geisler Norbert,
Plessmann Uwe,
Weber Klaus
Publication year - 1985
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/0014-5793(85)80357-4
Subject(s) - neurofilament , vimentin , intermediate filament , desmin , peptide sequence , sequence (biology) , protein filament , molecular mass , amino acid , amino acid residue , biology , protein sequencing , microbiology and biotechnology , biochemistry , cytoskeleton , chemistry , gene , cell , immunology , immunohistochemistry , enzyme
The first complete amino acid sequence of a neurofilament protein has been established. Porcine NF‐L contains 548 residues corresponding to a molecular mass of ~62 kDa. This value is noticeably smaller than the 68–72 kDa estimates from gel electrophoresis. Sequence comparison among the 6 non‐epithelial intermediate filament (IF) proteins of warm‐blooded vertebrates shows that the three NF proteins are the most remote members. Additionally and unexpectedly they reveal among each other lower sequence identity than the three non‐neuronal IF proteins GFAP, desmin, and vimentin where the last two are particularly closely related. Certain schemes of IF protein evolution are discussed.